Generator

Part:BBa_I766655:Design

Designed by: alexander ng   Group: iGEM07_UCSF   (2007-08-16)


YopJ-EE under medium constitutive promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 614
    Illegal NotI site found at 2054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1270
    Illegal BamHI site found at 1111
    Illegal BamHI site found at 2037
    Illegal XhoI site found at 254
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1039
    Illegal SapI.rc site found at 1063


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.


Source

composite part

References